Rose -
All For Drug Testing -
Fat BurnersSubject: Oxidative Stress-induced Insulin Resistance
Author: ironjustice@aol.comDate: 26 Sep 2008
Oxidative stress-induced insulin resistance in skeletal muscle cells
is ameliorated by gamma-tocopherol treatment.
Singh I, Carey AL, Watson N, Febbraio MA, Hawley JA
Eur J Nutr 2008 Sep 18.
BACKGROUND:
Oxidative stress-induced reactive oxygen species are associated with
the clinical manifestation of insulin resistance.
Evidence suggests that antioxidant treatment may reduce this
incidence.
AIM OF THE STUDY:
This study determined whether glucose oxidase (GO)-induced insulin
resistance in cultured skeletal muscle cells could be ameliorated by
pre-treatment with gamma-tocopherol (GT).
METHODS:
Insulin sensitivity in L6 myotubes was assessed by 2-deoxy-D: -[(3)H]-
glucose uptake.
The phosphorylation of distal insulin signaling proteins Akt and the
Akt substrate AS160 were determined by western blot.
RESULTS:
One hour treatment with 100 mU/ml GO decreased insulin-stimulated
glucose uptake (P < 0.001).
Pre-treatment with GT either partially (100 microM) or completely (200
microM) restored insulin-stimulated glucose uptake in cells after GO-
induced insulin resistance.
GO-induced oxidative stress did not impair insulin stimulated
phosphorylation of Akt or AS160, but 200 microM GT increased insulin-
stimulated phosphorylation of these key signaling proteins (P <
0.05).
CONCLUSIONS:
High-dose (200 microM) GT treatment ameliorated oxidative stress-
induced insulin resistance in cultured rat L6 skeletal muscle cells.
European journal of nutrition [Eur J Nutr]
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